Shenyang Automation Institute uses optical-electric coupling control mechanism to obtain cell membrane capacitance and


Acquiring cell intrinsic electrical eigen information experimental process

Recently, the micro-nano research group of the Shenyang Institute of Automation, Chinese Academy of Sciences has used micro-nano manipulation to obtain the latest research results in the field of cell information acquisition. The relevant research results are based on the determination of cell membrane capacitance and conductance via optically induced electrokinetics, published as cover papers. Biophysical Journal.

The life activities of the human body are all coordinated with the unit of cells. All life phenomena are closely related to the cell's growth, development, metabolism, and other cell intrinsic information. The acquisition of cellular intrinsic information has become increasingly important in biological research. The more important it is. The biochemical method is used to obtain the cell's intrinsic information, which can evaluate the function status of cells and provide scientific basis for the research and application of life science, disease medical diagnosis and drug development. However, the detection cost is high, the process is complicated, and the cells are damaged. Therefore, how to achieve the non-invasive acquisition of cellular intrinsic information is a difficult point in the field of biomedicine and bioengineering. In response to this problem, the micro-nano research group of the Shenyang Institute of Automation, Chinese Academy of Sciences carried out research work on the acquisition of the information on the electrical properties of cells and achieved initial results.

The dissertation discusses the research work of using optical-electric coupling control mechanism to obtain the cell membrane capacitance and membrane conductance. In combination with light-induced electrohydraulic dynamics, a cell mechanics model was precisely established, and large-scale, label-free, non-invasive acquisition of electrical eigen-information information for cancer cells was achieved. Quantitative characterization of drug concentrations acting on cancer cells was achieved through membrane capacitance parameters. . The new method proposed in this study is expected to provide new technical support for research on stem cell differentiation, cell life cycle, and cell surgery.

The research work was supported by the National Natural Science Foundation of China, the Chinese Academy of Sciences and the State Key Laboratory of Robotics.

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