The data obtained by measuring the biopesticide of the drug under the prescribed control conditions are calculated as indicators indicating the virulence of the drug, and the indicators are as follows.
(1) Lethal dose: The amount of the agent required to cause 50% of the individuals in the test organism to die, expressed by LD50. There are two types of dosage units: one is the unit quantity received by the unit mass of the test organism, such as mg/kg or microgram/g; the other is that the dose of the test organism is acceptable. Unit, such as the amount of milligrams (mg/piece) accepted by each individual, or the amount of micrograms (micrograms per unit) accepted by each individual.
(2) Fatal concentration: The concentration of the agent that can cause 50% of the individuals in the test organism to die, expressed by LC50. The concentration of the drug is a percentage of the active ingredient of the drug (generally in mass), a ratio of parts per thousand to a percentage of the drug, or 1%, 0.1%, etc., in the unit or unit mass of the drug.
(3) Effective medium dose: The amount of the agent required to produce a certain pharmacodynamic reaction in 50% of the individuals in the test organism population, expressed by ED50. A certain pharmacodynamic reaction means that the test organism can produce any abnormal reaction performance, such as insects being knocked down, losing activity, losing weight, stopping feeding, dying, spores not germinating, slow growth of mycelium, seeds Loss of budding power, slow growth and even dying, plant leaves green, leaf curl, and dead spots.
(4) Effective medium concentration: The concentration of the agent required to produce a certain medicinal response in 50% of the individuals in the test organism population, expressed as EC50.
(5) Relative virulence index: When comparing the virulence of multiple agents, it is sometimes necessary to perform virulence determination in batches. Due to the difference between the internal factors of the test organism and the processing conditions during the measurement, different batches are caused. The test results of the second time have a certain degree of change. In order to eliminate the influence of the above differences, a pesticide is selected as a standard agent, and the ratio of each test agent to its virulence is determined. This ratio to the standard agent is called the relative virulence index. The relative virulence index was calculated by the following formula.
Relative virulence index = (equivalent dose of standard drug ç‰æ•ˆ equivalent dose of other drugs) × 100
The equivalent dose is the dose (or concentration) required for two or more agents to produce the same size response to the test organism under the same test conditions. Usually a lethal dose is used, and a 90% dose (LD90) or other lethal dose can be used. For example, the lethal doses of A and B for a certain pest are 5 mg/g and 3 μg/g, respectively. When the drug is used as a standard drug and the relative virulence index is 100, the relative virulence index of the drug is for:
Relative toxicity index of a drug (=3÷5)×100=60
The greater the relative virulence index, the greater the virulence of the agent. The relative virulence index can be used to sequence the virulence of the bioassay.
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